| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3141 | Thermotoga | TCCAGGTACCCAGAAGCAGT | ATTCGCGACAGCCGACTT | 60.18 | 59.74 | 248 | 47 |
100.00%
|
80.00%
|
| Primer 3142 | Thermotoga | ACAGGGCAAGACTCTTCGC | ACACACGTCTCCTGCCTT | 60.00 | 58.45 | 158 | 47 |
100.00%
|
60.00%
|
| Primer 3143 | Thermotoga | TGAAGGTGGTTCAGGACGC | TGTTGGCGTGATCTCCCAC | 59.93 | 60.00 | 216 | 47 |
100.00%
|
80.00%
|
| Primer 3144 | Thermotoga | CGCCACGCAAAATCCGAT | ATCGGCTTCAGCTGGCTT | 59.21 | 59.33 | 165 | 47 |
100.00%
|
40.00%
|
| Primer 3145 | Thermotoga | ATCGCCACGCAAAATCCG | ATCGGCTTCAGCTGGCTT | 59.21 | 59.33 | 167 | 47 |
100.00%
|
40.00%
|
| Primer 3146 | Thermotoga | TCAGGCTGTAACCGTTGCA | TTCGGCATTCGCACCAGA | 59.55 | 59.65 | 214 | 47 |
100.00%
|
40.00%
|
| Primer 3147 | Thermotoga | AGCGCTTTGTCGAAACCG | ACACCGTCACCTTCACGAC | 59.06 | 59.93 | 228 | 46 |
100.00%
|
60.00%
|
| Primer 3148 | Thermotoga | ACCTTCTGGGCTGTCATGC | AAAGGGTTCAGCGCACCA | 60.00 | 59.81 | 217 | 46 |
100.00%
|
40.00%
|
| Primer 3149 | Thermotoga | ACAGCCAGAAGAACGGTCG | CGTGGAACTCCGCTTTTGC | 60.01 | 60.08 | 259 | 46 |
100.00%
|
60.00%
|
| Primer 3150 | Thermotoga | ACAGCCAGAAGAACGGTCG | GCGTGGAACTCCGCTTTTG | 60.01 | 60.08 | 260 | 46 |
100.00%
|
60.00%
|