| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3211 | Thermotoga | AGGCCAGAAGTGCACGAA | TGGCGTTCTGTCTTGCGT | 59.17 | 59.89 | 181 | 45 |
100.00%
|
60.00%
|
| Primer 3212 | Thermotoga | ACAGAGGCCAGAAGTGCAC | TGGCGTTCTTCATGGCGT | 59.93 | 59.97 | 197 | 45 |
100.00%
|
80.00%
|
| Primer 3213 | Thermotoga | ACAGAGGCCAGAAGTGCAC | TGGCGTTCTGTCTTGCGT | 59.93 | 59.89 | 185 | 45 |
100.00%
|
80.00%
|
| Primer 3214 | Thermotoga | AACAGCGAGAAGAAGGCGT | ACGTCGTTCGAACCGGTT | 59.63 | 59.59 | 269 | 45 |
100.00%
|
80.00%
|
| Primer 3215 | Thermotoga | CGTCACGGGTTCTCAGCTT | TCAGTGAAGACGCGACGT | 60.01 | 58.97 | 257 | 45 |
100.00%
|
80.00%
|
| Primer 3216 | Thermotoga | AGTGTCGATGCAGCCCAT | TCACGAACACCACGTCGT | 59.01 | 59.20 | 238 | 45 |
100.00%
|
40.00%
|
| Primer 3217 | Thermotoga | AAAGTGTCGATGCAGCCCA | TCACGAACACCACGTCGT | 59.93 | 59.20 | 240 | 45 |
100.00%
|
80.00%
|
| Primer 3218 | Thermotoga | AGTGTCGATGCAGCCCATC | TCACGAACACCACGTCGT | 60.15 | 59.20 | 238 | 45 |
100.00%
|
40.00%
|
| Primer 3219 | Thermotoga | TCAAACCTCCAACCTGCAGG | TCCTCGGGAACAGGATCGT | 60.18 | 60.00 | 157 | 45 |
100.00%
|
80.00%
|
| Primer 3220 | Thermotoga | CGCAGAGCCGATTCTTGGA | CGTCCCAGCCCTTCACATT | 60.15 | 60.00 | 176 | 45 |
100.00%
|
60.00%
|