| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3231 | Thermotoga | AGAGTCTGGGAAGGCACCT | CTGGCCAGGTGTGAATGGT | 59.84 | 59.92 | 210 | 44 |
100.00%
|
100.00%
|
| Primer 3232 | Thermotoga | TTCGATGTGGTGGAAGGGC | AAGGTGCGTCGCATCGTA | 60.00 | 59.43 | 237 | 44 |
100.00%
|
80.00%
|
| Primer 3233 | Thermotoga | ACTCGCCTCCAGCATGTT | AGGACTGTTGGAACGGCTG | 58.93 | 59.93 | 180 | 44 |
100.00%
|
80.00%
|
| Primer 3234 | Thermotoga | GAGGTGTTGATGCTGGCCT | TCTGTTTCCCGTGTGGCA | 60.00 | 59.09 | 225 | 44 |
100.00%
|
80.00%
|
| Primer 3235 | Thermotoga | ACCTCCCTCATGATCGCCT | ACCTGCGAGTGCTTCTTCC | 60.07 | 60.00 | 165 | 44 |
100.00%
|
80.00%
|
| Primer 3236 | Thermotoga | ACCTCCCTCATGATCGCCT | CCTGCGAGTGCTTCTTCCA | 60.07 | 60.00 | 164 | 44 |
100.00%
|
80.00%
|
| Primer 3237 | Thermotoga | ACCTCCCTCATGATCGCCT | ATGGATGCCCACACGTCTG | 60.07 | 60.08 | 200 | 44 |
100.00%
|
100.00%
|
| Primer 3238 | Thermotoga | AGGTGGGCAGCTTCAAAGG | TGACTCCCAGCTGATCCCT | 60.23 | 59.61 | 152 | 44 |
100.00%
|
40.00%
|
| Primer 3239 | Thermotoga | TCAGGTGGGCAGCTTCAA | TGACTCCCAGCTGATCCCT | 58.75 | 59.61 | 154 | 44 |
100.00%
|
40.00%
|
| Primer 3240 | Thermotoga | AGGACGCTTTCAAGGCCT | TGTTGGCGTGATCTCCCAC | 58.84 | 60.00 | 204 | 44 |
100.00%
|
80.00%
|