| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3221 | Thermotoga | CCGGAAGAGGTGGCAGAAA | CGTCCCAGCCCTTCACATT | 59.63 | 60.00 | 247 | 45 |
100.00%
|
40.00%
|
| Primer 3222 | Thermotoga | TGGGATCGTAACGGCAGAC | TCCACCAGGGATGGATCGT | 59.49 | 60.00 | 297 | 45 |
100.00%
|
40.00%
|
| Primer 3223 | Thermotoga | GTGGGATCGTAACGGCAGA | TCCACCAGGGATGGATCGT | 59.49 | 60.00 | 298 | 45 |
100.00%
|
40.00%
|
| Primer 3224 | Thermotoga | AGACTCCACGGTCACACCT | TGGGCTGGTCGAACATGTC | 60.15 | 60.00 | 160 | 44 |
100.00%
|
80.00%
|
| Primer 3225 | Thermotoga | GAGAGCGCAGGAAGGTTGT | ACCTCTTCGGTCACCACCT | 60.00 | 60.15 | 265 | 44 |
100.00%
|
80.00%
|
| Primer 3226 | Thermotoga | AGAGCGCAGGAAGGTTGT | ACCTCTTCGGTCACCACCT | 58.85 | 60.15 | 264 | 44 |
100.00%
|
40.00%
|
| Primer 3227 | Thermotoga | CGGAAGATGTTCGAGCCCA | ACGCAATCCACGTGAGGTT | 59.78 | 59.93 | 161 | 44 |
100.00%
|
80.00%
|
| Primer 3228 | Thermotoga | AAGTCCCTCCGAGTGTCCT | TCGCGAAAACACCGTGCT | 59.53 | 60.59 | 200 | 44 |
100.00%
|
40.00%
|
| Primer 3229 | Thermotoga | CGTTCTTTGCACGGCACA | ACGCTGCCTTTGCTACCT | 59.29 | 59.25 | 218 | 44 |
100.00%
|
80.00%
|
| Primer 3230 | Thermotoga | TCTGGACAACGTTTGCGGT | ACCCCTCATGGCGTATCCT | 60.15 | 60.07 | 223 | 44 |
100.00%
|
80.00%
|