| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3251 | Thermotoga | ACGCTGCGGAATTCGTGA | ACACACCACCGCTTGCTA | 60.05 | 59.17 | 198 | 44 |
100.00%
|
40.00%
|
| Primer 3252 | Thermotoga | GACCGTCTGGCTGTGGAAA | ACCCTCTGCTTCATCCCCT | 59.93 | 59.92 | 262 | 44 |
100.00%
|
80.00%
|
| Primer 3253 | Thermotoga | ACCGTCTGGCTGTGGAAA | ACCCTCTGCTTCATCCCCT | 58.76 | 59.92 | 261 | 44 |
100.00%
|
80.00%
|
| Primer 3254 | Thermotoga | ACAGGGCAAGACTCTTCGC | AACACACGTCTCCTGCCT | 60.00 | 58.45 | 159 | 44 |
100.00%
|
60.00%
|
| Primer 3255 | Thermotoga | CCCGTACGGTGAGACGAAA | GCTGAAACCACCGAGTCCT | 59.42 | 59.63 | 151 | 44 |
100.00%
|
80.00%
|
| Primer 3256 | Thermotoga | TTCGATGTGGTGGAAGGGC | AAGGTGCGTCGCATCGTA | 60.00 | 59.43 | 237 | 44 |
100.00%
|
80.00%
|
| Primer 3257 | Thermotoga | ACTGCCGTTGGACTCGTT | AAGGGGAATGATGCGGTCG | 59.18 | 60.15 | 243 | 44 |
100.00%
|
80.00%
|
| Primer 3258 | Thermotoga | ACTGCCGTTGGACTCGTT | GGGGAATGATGCGGTCGAA | 59.18 | 60.15 | 241 | 44 |
100.00%
|
80.00%
|
| Primer 3259 | Thermotoga | TGCGCAGGACACGGAAAT | ACCTCTTCGGTCACCACCT | 59.97 | 60.15 | 231 | 44 |
100.00%
|
80.00%
|
| Primer 3260 | Thermotoga | ATTGCGCAGGACACGGAA | ACCTCTTCGGTCACCACCT | 59.97 | 60.15 | 233 | 44 |
100.00%
|
80.00%
|