| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3271 | Thermotoga | AGGACGCTTTCAAGGCCT | TGTTGGCGTGATCTCCCAC | 58.84 | 60.00 | 204 | 44 |
100.00%
|
80.00%
|
| Primer 3272 | Thermotoga | AGGTGGGAAAGTCGCGTT | TGAACCCTCTGCGCTGTT | 59.17 | 59.17 | 243 | 44 |
100.00%
|
40.00%
|
| Primer 3273 | Thermotoga | TCGCGTTCAAAGGATGGCT | TGAACCCTCTGCGCTGTT | 60.00 | 59.17 | 232 | 44 |
100.00%
|
80.00%
|
| Primer 3274 | Thermotoga | GGTCCTCGATCTTGCCACA | TCTGAAGCCGAAAGCCACG | 59.40 | 60.67 | 214 | 43 |
100.00%
|
80.00%
|
| Primer 3275 | Thermotoga | TGAATGAGACGCTCGCCA | GCGTCCTGAACCACCTTCA | 59.03 | 59.93 | 292 | 43 |
100.00%
|
60.00%
|
| Primer 3276 | Thermotoga | TGAATGAGACGCTCGCCAG | GCGTCCTGAACCACCTTCA | 60.15 | 59.93 | 292 | 43 |
100.00%
|
60.00%
|
| Primer 3277 | Thermotoga | AGATCCCCACAGGCTCGAT | CCAGCTTCAACCGCTCTCA | 60.07 | 60.00 | 174 | 43 |
100.00%
|
60.00%
|
| Primer 3278 | Thermotoga | CATGGCCAGGAGTTCGTGA | AGTTCCGGAAAGAGCGCA | 59.70 | 59.26 | 203 | 43 |
100.00%
|
60.00%
|
| Primer 3279 | Thermotoga | ACGGAATGCTCGCCATGA | AGCAAGGAACTCCACCGTG | 59.42 | 59.93 | 155 | 43 |
100.00%
|
40.00%
|
| Primer 3280 | Thermotoga | TAGGTCGCAACGTGGTGA | CCTCAGTCACGGCAGATGT | 58.55 | 59.41 | 171 | 43 |
100.00%
|
80.00%
|