| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3261 | Thermotoga | TGCAAAAGCCGAGCCTGT | CCTGCACTCAAGCCAGCTA | 60.20 | 59.70 | 172 | 44 |
100.00%
|
60.00%
|
| Primer 3262 | Thermotoga | ACCGTGCCTCGTCTTTCA | CACCACCGTATCACCCGTT | 58.87 | 59.71 | 285 | 44 |
100.00%
|
40.00%
|
| Primer 3263 | Thermotoga | CCGTGCCTCGTCTTTCACA | CACCACCGTATCACCCGTT | 60.30 | 59.71 | 284 | 44 |
100.00%
|
80.00%
|
| Primer 3264 | Thermotoga | ACCGTGCCTCGTCTTTCAC | CACCACCGTATCACCCGTT | 60.30 | 59.71 | 285 | 44 |
100.00%
|
80.00%
|
| Primer 3265 | Thermotoga | AGTGTGGAAGCGGTGGTT | TCCTGGAGCATTTGGGCAG | 59.08 | 60.00 | 194 | 44 |
100.00%
|
80.00%
|
| Primer 3266 | Thermotoga | AGTGTGGAAGCGGTGGTT | CTCCTGGAGCATTTGGGCA | 59.08 | 60.00 | 195 | 44 |
100.00%
|
80.00%
|
| Primer 3267 | Thermotoga | AACGAAGCGCTCCAGATGA | CGAGCTCGTTCATTTCCCCT | 59.41 | 60.11 | 163 | 44 |
100.00%
|
80.00%
|
| Primer 3268 | Thermotoga | AAGTGTCGATGCAGCCCA | TCACGAACACCACGTCGTT | 59.25 | 59.86 | 239 | 44 |
100.00%
|
40.00%
|
| Primer 3269 | Thermotoga | AAGTCGTTGCTCGCACCT | ATGGCCAGCACTTTCGCA | 59.58 | 60.28 | 155 | 44 |
100.00%
|
60.00%
|
| Primer 3270 | Thermotoga | ACAGGGCAAGACTCTTCGC | AACACACGTCTCCTGCCT | 60.00 | 58.45 | 159 | 44 |
100.00%
|
60.00%
|