| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3281 | Thermotoga | GGTCCTCGATCTTGCCACA | TCTGAAGCCGAAAGCCACG | 59.40 | 60.67 | 214 | 43 |
100.00%
|
80.00%
|
| Primer 3282 | Thermotoga | CATGGCCAGGAGTTCGTGA | AGTTCCGGAAAGAGCGCA | 59.70 | 59.26 | 203 | 43 |
100.00%
|
60.00%
|
| Primer 3283 | Thermotoga | AAGACGCCTTCACGCTGA | TCCGGTGAAAACCACTCCG | 59.27 | 59.93 | 176 | 43 |
100.00%
|
60.00%
|
| Primer 3284 | Thermotoga | TTCCTGCCACACCAGCAT | CCAAGCTCCGCTTCCATCA | 59.15 | 60.08 | 279 | 43 |
100.00%
|
80.00%
|
| Primer 3285 | Thermotoga | AGTGGCTCCTCGAGGATGA | CGGCAACAAGCTGTGCAT | 59.69 | 59.35 | 264 | 43 |
100.00%
|
80.00%
|
| Primer 3286 | Thermotoga | AACTTCCCAGCCGAGTGTG | GCATTTCACGAGCCACACG | 59.93 | 60.15 | 221 | 43 |
100.00%
|
80.00%
|
| Primer 3287 | Thermotoga | TGAATGAGACGCTCGCCA | GCGTCCTGAACCACCTTCA | 59.03 | 59.93 | 292 | 43 |
100.00%
|
60.00%
|
| Primer 3288 | Thermotoga | TGAATGAGACGCTCGCCAG | GCGTCCTGAACCACCTTCA | 60.15 | 59.93 | 292 | 43 |
100.00%
|
60.00%
|
| Primer 3289 | Thermotoga | TGCTGTTGTACTCGCGTCA | ATTGCCATCGTTGCGAGC | 59.64 | 59.21 | 156 | 43 |
100.00%
|
40.00%
|
| Primer 3290 | Thermotoga | ATCACGCCTCCTGCAGAA | TTCGAGCGATCTTACACCGA | 58.61 | 58.91 | 199 | 42 |
100.00%
|
80.00%
|