| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Methanosphaera | TCTTGCAGGAGATCAGCCAAC | GGGCATACCTAAACCTTCAGC | 60.34 | 58.70 | 151 | 151 |
100.00%
|
66.67%
|
| Primer 2 | Methanosphaera | GGGATCAGTTGCAAAGGGC | TGTACCAATTCCTCCTGGTGT | 59.11 | 58.66 | 193 | 143 |
100.00%
|
66.67%
|
| Primer 3 | Methanosphaera | TGGGATCAGTTGCAAAGGGC | TGTACCAATTCCTCCTGGTGT | 60.90 | 58.66 | 194 | 143 |
100.00%
|
66.67%
|
| Primer 4 | Methanosphaera | ATGGGATCAGTTGCAAAGGGC | TGTACCAATTCCTCCTGGTGT | 61.24 | 58.66 | 195 | 143 |
100.00%
|
66.67%
|
| Primer 5 | Methanosphaera | GCGTAAGGCATGTTATGAGGA | TCACCACCACAGGATAGGGC | 58.17 | 61.27 | 177 | 140 |
100.00%
|
66.67%
|
| Primer 6 | Methanosphaera | GCATGGGCACAAGTTGATGT | TCAGCAACACCCTGTGCA | 59.40 | 59.41 | 155 | 138 |
100.00%
|
33.33%
|
| Primer 7 | Methanosphaera | GCGTAAGGCATGTTATGAGGA | CCACCACAGGATAGGGCTAG | 58.17 | 58.95 | 174 | 138 |
100.00%
|
66.67%
|
| Primer 8 | Methanosphaera | GCGTAAGGCATGTTATGAGGA | ACCACCACAGGATAGGGCTAG | 58.17 | 60.69 | 175 | 138 |
100.00%
|
66.67%
|
| Primer 9 | Methanosphaera | GCATGGGCACAAGTTGATGT | TCAGCAACACCCTGTGCAT | 59.40 | 59.85 | 155 | 137 |
100.00%
|
33.33%
|
| Primer 10 | Methanosphaera | GCATGGGCACAAGTTGATGT | CAGCAACACCCTGTGCAT | 59.40 | 58.23 | 154 | 137 |
100.00%
|
33.33%
|