| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Ralstonia | ATCGTTGCTGCCCTGCAT | GCGTACGTTGAACTGCGTG | 60.04 | 60.15 | 195 | 149 |
100.00%
|
33.33%
|
| Primer 2 | Ralstonia | TTCTCCCGAGTCCAGCGAT | GGGCTTTTCGCTTTCCAGC | 60.38 | 60.08 | 184 | 141 |
100.00%
|
33.33%
|
| Primer 3 | Ralstonia | ACTGAACGTGACGCTGCA | TTAGGCGTTGACAGCGTCC | 59.89 | 60.37 | 205 | 138 |
100.00%
|
16.67%
|
| Primer 4 | Ralstonia | TGCTGCAACCTAAGCGCA | ATGTGACGGGTCATCGCA | 60.28 | 59.02 | 172 | 138 |
100.00%
|
16.67%
|
| Primer 5 | Ralstonia | TGCTGCAACCTAAGCGCA | TTCGGCAGGCTTCTTCGAG | 60.28 | 60.08 | 239 | 138 |
100.00%
|
16.67%
|
| Primer 6 | Ralstonia | TGCTGCAACCTAAGCGCA | CGGCAGGCTTCTTCGAGAT | 60.28 | 59.86 | 237 | 138 |
100.00%
|
16.67%
|
| Primer 7 | Ralstonia | TGCGTGCCATGTTTGCTC | TGCCGTCGTTGTTGGTGT | 59.36 | 60.13 | 297 | 137 |
100.00%
|
16.67%
|
| Primer 8 | Ralstonia | CTGATGTTGGCGACGCTTG | TGCCGTCGTTGTTGGTGT | 59.87 | 60.13 | 259 | 137 |
100.00%
|
16.67%
|
| Primer 9 | Ralstonia | ATGCGTGCCATGTTTGCTC | TGCCGTCGTTGTTGGTGT | 59.79 | 60.13 | 298 | 137 |
100.00%
|
16.67%
|
| Primer 10 | Ralstonia | TGATGTTGGCGACGCTTG | TGCCGTCGTTGTTGGTGT | 58.75 | 60.13 | 258 | 137 |
100.00%
|
16.67%
|